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A. Definitions of Transformation in vitro When normal tissues or organs are explanted to conditions favoring the growth of cells as individual units ("cell culture").
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This simplifies analysis of the biology of cells which may otherwise have a limited lifetime. Immortalised cell lines can also be cloned giving rise to a clonal population which can, in turn, be propagated indefinitely.

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This allows an analysis to be repeated many times on genetically identical cells, which is desirable for repeatable scientific experiments. The alternative, performing an analysis on primary cells from multiple tissue donors, does not have this advantage.

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  5. Immortalised cell lines find use in biotechnology, where they are a cost-effective way of growing cells similar to those found in a multicellular organism in vitro. The cells are used for a wide variety of purposes, from testing toxicity of compounds or drugs to production of eukaryotic proteins. While immortalised cell lines often originate from a well-known tissue type, they have undergone significant mutations to become immortal.

    This can alter the biology of the cell and must be taken into consideration in any analysis.

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    Further, cell lines can change genetically over multiple passages, leading to phenotypic differences among isolates and potentially different experimental results depending on when and with what strain isolate an experiment is conducted. Many cell lines that are widely used for biomedical research have been contaminated and overgrown by other, more aggressive cells. For example, supposed thyroid lines were actually melanoma cells, supposed prostate tissue was actually bladder cancer, and supposed normal uterine cultures were actually breast cancer.

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    There are several methods for generating immortalised cell lines: [4]. There are several examples of immortalised cell lines, each with different properties.

    Cell culture techniques 2 - Isolation of cell lines for in vitro culture

    Most immortalised cell lines are classified by the cell type they originated from or are most similar to biologically. From Wikipedia, the free encyclopedia. Ebooks and eBook viewers provide substantial benefits more than traditional reading. Ebooks reduce down on the employ of paper, as recommended by environmental enthusiasts. There are no fixed timings for study. There is no question of waiting-time for new editions.

    Immortalised cell line

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    Not merely that, the online variation of books are typically cheap, because publication homes save their print and paper machinery, the benefits of which are given to to customers. With most organs fibroblast-like cells survive longest and outgrow other types. This treatise will not discuss whether this simplification and stabilization represents selection of certain pre-existing cell types or a modification of cells into only a few recognizably different categories; for an excellent review see HARRIS.

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    Table 1. Such changes have, sometimes rather vaguely, been called "transformations".

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    This is unprecise and the term "transformation" will here be used exclusively to indicate disturbances in cell growth related to neoplasia.